Nick Mallet has some key injuries which will make the job in Wales on the 23 February even harder.
Full-back David Bortolussi injured his shoulder in training with his French club Montpellier and could miss the rest of the Six Nations, whilst wing Kaine Robertson is out with a thigh injury.
The other changes are: Alessio Galante replaces Ludovico Nitoglia, who was a late call-up, while former captain Marco Bortolami also returns along with uncapped 21-year-old full-back Paolo Buso.
Squad to face Wales on 23 February:
Mauro Bergamasco, Mirco Bergamasco, Marco Bortolami, Paolo Buso, Gonzalo Canale, Martin Castrogiovanni, Carlo Antonio Del Fava, Santiago Dellape, Carlo Festuccia, Ezio Galon, Leonardo Ghiraldini, Andrea Lo Cicero, Andrea Marcato, Andrea Masi, Carlos Nieto, Sergio Parisse (captain), Enrico Patrizio, Salvatore Perugini, Simon Picone, Alessio Galante, Alberto Sgarbi, Josh Sole, Pietro Travagli, Alessandro Zanni